The Seminoles (5-2) dominated from the opening tip, taking a 25-9 lead and never letting the Gators (5-1) back in the game.
The two-time defending national champions had their 15-game winning streak snapped along with their school-record 24-game home winning streak. It was Florida's worst loss since falling 75-60 to Manhattan in the first round of the 2004 NCAA tournament.
Florida State had lost two of its last three games, surprisingly falling to Cleveland State and South Florida in the past week. But Hamilton's team hardly looked the same in the O'Connell Center, where FSU won for the first time since 1992.
NC STATE 63, South Carolina 61
MARYLAND 72, Lehigh 51
WAKE FOREST 73, Winston-Salem State 53
VIRGINIA 100, Penn 85
Butler 84, VIRGINIA TECH 78 (OT)
NORTH CAROLINA 99, Old Dominion 82
(AP Photos/Phil Sandlin and Jae C. Hong)